Composite
Part:BBa_K2398538:Design
Designed by: Thore Buergel, Lukas Adam, Catharina Gandor, Marita Klein, Jan Mathony, Pauline Pfuderer, Lukas Platz, Moritz Przybilla, Max Schwendemann, Julius Upmeier zu Belzen Group: iGEM17_Heidelberg (2017-10-26)
beta-lactamase_M67G_G216C_P217F
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
This part contains a fully functional beta-lactamase expression cassette. E. coli beta-lactamase is under control of the constitutive wildtype E. coli promoter. Expression is terminated by the his-operon terminator of pSB1C3.
In this part and related parts, mutations are introduced into the coding sequence. These mutations modify the enzyme activity (see results).
Design Notes
compatible with RFC10
Source
genomic with modification